Pros and cons of biotechnology using mammalian expression systems
In biotechnology, mammalian protein expressions refers to the method of transfecting genes into bovines to create fully folded proteins. End customers can grow their own monoclonal antibodies across various cell types (hybridomas, human cell lines, CHO, etc.), or they can partner with contract manufacturing organizations that can deliver loads of recombinant proteins of a good variety. of sources.
Advantages of using bovine protein expressions
The presence of subsequent modifications to the gene is considered to produce the greatest functionality. The resulting recombinant proteins can be further modified by post-transnational procedures in bovine cells. Cytotoxic antibody conjugates through mammalian expression have also been found to be more compatible with humans. Similarly, they also have very low immunogenicity and high immunity to human pathogens. In short, they are very safe for human use. All of these factors mean that the path to gaining permission from regulatory bodies is much easier when going through the mammalian route of expression.
The Cons of Mammalian Expression
Unfortunately, no method is perfect. This is particularly true with recombinant proteins where integration with various cell types can produce various results along with their consequences. In the case of bovine expression, it is quite difficult to initiate transfection of the gene of interest into the bovine cell. Furthermore, single clone extraction is a very laborious process when compared to other expression methods such as bacterial, insect cell, yeast, eukaryotic plant and cell-free systems. These are just some of the contributing factors as to why the production rate using mammalian expression remains low. And this is the reason why protein culture and monoclonal antibody production using bovine expression is quite expensive. To circumvent the time-consuming process, biotech companies can partner with contract manufacturing organizations (CMOs) that can meet their requirements.
CMO Election
It is true that the large number of CMOs makes it difficult to choose the ideal partner. A good tip is to look for biologics manufacturing companies that earn the respect of your peers and experts. The CMO must be able to offer solutions to even the most difficult problems facing them, covering a full range of services including Biobreeders, Biosimilars, Cell Line and Cell Bank Development, Transgenics, GMP Manufacturing, Bioconjugation, Fill and Finish aseptic and consulting. services. A good way to start is at the top, which means you choose from Life Science Leaders magazine’s annual CMO Leadership Award winners.